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rabbit anti n wasp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti n wasp
    Rabbit Anti N Wasp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+wasp/N-WASP+Rabbit+mAb/pm41360804-416-49-51
    Average 95 stars, based on 125 article reviews
    rabbit anti n wasp - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: <i>LINC00869</i> Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation
    Article Snippet: Coordination of filament assembly andmembrane remodeling is required for the directional migration of cancer cells.. The Wiskott– Aldrich syndrome protein (WASP) recruits the actin-related protein (ARP) 2/3 complex to assemble branched actin networks.. The goal of our study was to assess the potential regulatory role exerted by the novel long noncoding RNA (lncRNA) LINC00869 on hepatocellular carcinoma (HCC) cells.We usedHCC cells to overexpress or knockdown LINC00869, analyzed patient data from publicly available databases and Cancer Hospital Affiliated with Zhengzhou University, and used a xenograft mouse model of HCC to study the molecular mechanism associated with LINC00869 expression.

    Article Title: Small GTPase Cdc42, WASP, and scaffold proteins for higher-order assembly of the F-BAR domain protein.
    Article Snippet: .. The membranes were incubated with the primary antibody: mouse anti-GAS7 (clone 2F6, TA501756, OriGene), anti-Grb2 (Sc-8034, OriGene), anti-Nck (SC-20026, Santa Cruz Biotechnology), anti–N-WASP (#4848, Cell Signaling Technology), anti-WASP (#48606, Cell Signaling Technology), anti-SPIN90 (Ab88467, Abcam), anti-mCherry (71615, Cell Signaling Technology), and anti–glyceraldehyde-3-phosphate dehydrogenase (SC16657, Santa Cruz Biotechnology) at a 1:10,000 dilution, followed by an anti-mouse or anti-rabbit IgG alkaline phosphatase conjugate (Promega) secondary antibody in PBS-T at a 1:10,000 dilution. .. The alkaline phosphatase was detected by 5-bromo-4chloro-3-indolyl phosphate/nitro blue tetrazolium (Roche).

    other:

    Article Title: Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction
    Article Snippet: Anti-WASp , , – , 4860 , Cell Signalling Technology.

    Article Title: Arpin deficiency increases actomyosin contractility and vascular permeability
    Article Snippet: Antibodies against WASP, WAVE2 (clone DSC8), p-Cofilin (S3) (clone 77G2), cofilin, pMLC (Ser19), MLC, pMYPT1 (Thr696), pMYPT1 (Thr 853), MYPT1, ROCK1 (clone C8F7), and DAPK3/ZIPK were purchased from Cell Signaling Technology (Danvers, MA).

    Article Title: Arpin deficiency increases actomyosin contractility and vascular permeability
    Article Snippet: Antibodies against WASP, WAVE2 (clone DSC8), p- Cofilin (S3) (clone 77G2), cofilin, pMLC (Ser19), MLC, pMYPT1 (Thr696), pMYPT1 (Thr 853), MYPT1, ROCK1 (clone C8F7), and DAPK3/ZIPK were purchased from Cell Signaling Technology (Danvers, MA).

    Article Title: Arpin deficiency increases actomyosin contractility and vascular permeability
    Article Snippet: Antibodies against WASP, WAVE2 (clone DSC8), p-cofilin (S3) (clone 77G2), cofilin, pMLC (Ser19), MLC, pMYPT1 (Thr696), pMYPT1 (Thr 853), MYPT1, ROCK1 (clone C8F7) and DAPK3/ZIPK were purchased from Cell Signaling Technology (Danvers, MA).

    Western Blot:

    Article Title: PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome assembly.
    Article Snippet: .. The primary antibodies included anti-WASP (Cell Signaling Technology 4860; 1:1000), anti-GFP (Cell Signaling Technology 2956; 1:1000), anti-PIK3CB (Proteintech 67121-1-Ig; 1:2000), anti-AKT (Cell Signaling Technology 4691; 1:1000), anti-Phospho-Akt (Ser473) (Cell Signaling Technology 4060; 1:1000), anti-VAV1 (Invitrogen MA531488; 1:1000 for western blotting, 1:100 for immunofluorescence), anti-Rac1 (BD Biosciences 610651; 1:1000), antiCdc42 (Proteintech 10155-1-AP; 1:1000) and anti-GAPDH (Thermo Fisher Scientific AM4300; 1:10000). .. The secondary antibodies used were anti-mouse HRP (Cell Signaling Technology 7076; 1:2000) and anti-rabbit HRP (Cell Signaling Technology 7074; 1:2000), AF594-anti-mouse (Thermo Fisher Scientific A-21203; 1:500), AF594-antirabbit (Thermo Fisher Scientific A-21207; 1:500), AF488anti-mouse (Thermo Fisher Scientific A-21202; 1:500), and AF488-anti-rabbit (Thermo Fisher Scientific A-21206; 1:500).

    Article Title: PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome assembly
    Article Snippet: .. The primary antibodies included anti-WASP (Cell Signaling Technology 4860; 1:1000), anti-GFP (Cell Signaling Technology 2956; 1:1000), anti-PIK3CB (Proteintech 67121-1-Ig; 1:2000), anti-AKT (Cell Signaling Technology 4691; 1:1000), anti-Phospho-Akt (Ser473) (Cell Signaling Technology 4060; 1:1000), anti-VAV1 (Invitrogen MA5-31488; 1:1000 for western blotting, 1:100 for immunofluorescence), anti-Rac1 (BD Biosciences 610651; 1:1000), anti-Cdc42 (Proteintech 10155-1-AP; 1:1000) and anti-GAPDH (Thermo Fisher Scientific AM4300; 1:10000). .. The secondary antibodies used were anti-mouse HRP (Cell Signaling Technology 7076; 1:2000) and anti-rabbit HRP (Cell Signaling Technology 7074; 1:2000), AF594-anti-mouse (Thermo Fisher Scientific A-21203; 1:500), AF594-anti-rabbit (Thermo Fisher Scientific A-21207; 1:500), AF488-anti-mouse (Thermo Fisher Scientific A-21202; 1:500), and AF488-anti-rabbit (Thermo Fisher Scientific A-21206; 1:500).

    Immunofluorescence:

    Article Title: PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome assembly.
    Article Snippet: .. The primary antibodies included anti-WASP (Cell Signaling Technology 4860; 1:1000), anti-GFP (Cell Signaling Technology 2956; 1:1000), anti-PIK3CB (Proteintech 67121-1-Ig; 1:2000), anti-AKT (Cell Signaling Technology 4691; 1:1000), anti-Phospho-Akt (Ser473) (Cell Signaling Technology 4060; 1:1000), anti-VAV1 (Invitrogen MA531488; 1:1000 for western blotting, 1:100 for immunofluorescence), anti-Rac1 (BD Biosciences 610651; 1:1000), antiCdc42 (Proteintech 10155-1-AP; 1:1000) and anti-GAPDH (Thermo Fisher Scientific AM4300; 1:10000). .. The secondary antibodies used were anti-mouse HRP (Cell Signaling Technology 7076; 1:2000) and anti-rabbit HRP (Cell Signaling Technology 7074; 1:2000), AF594-anti-mouse (Thermo Fisher Scientific A-21203; 1:500), AF594-antirabbit (Thermo Fisher Scientific A-21207; 1:500), AF488anti-mouse (Thermo Fisher Scientific A-21202; 1:500), and AF488-anti-rabbit (Thermo Fisher Scientific A-21206; 1:500).

    Article Title: PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome assembly
    Article Snippet: .. The primary antibodies included anti-WASP (Cell Signaling Technology 4860; 1:1000), anti-GFP (Cell Signaling Technology 2956; 1:1000), anti-PIK3CB (Proteintech 67121-1-Ig; 1:2000), anti-AKT (Cell Signaling Technology 4691; 1:1000), anti-Phospho-Akt (Ser473) (Cell Signaling Technology 4060; 1:1000), anti-VAV1 (Invitrogen MA5-31488; 1:1000 for western blotting, 1:100 for immunofluorescence), anti-Rac1 (BD Biosciences 610651; 1:1000), anti-Cdc42 (Proteintech 10155-1-AP; 1:1000) and anti-GAPDH (Thermo Fisher Scientific AM4300; 1:10000). .. The secondary antibodies used were anti-mouse HRP (Cell Signaling Technology 7076; 1:2000) and anti-rabbit HRP (Cell Signaling Technology 7074; 1:2000), AF594-anti-mouse (Thermo Fisher Scientific A-21203; 1:500), AF594-anti-rabbit (Thermo Fisher Scientific A-21207; 1:500), AF488-anti-mouse (Thermo Fisher Scientific A-21202; 1:500), and AF488-anti-rabbit (Thermo Fisher Scientific A-21206; 1:500).



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    a Representative brightfield and fluorescence images of HeLa cells treated with internalization inhibitors on AS1411 MP surfaces. b Corresponding quantification of mean fluorescence intensity per cell for HeLa cells. n = 70, 70, 54, 54 (left to right) cells from 3 replicates. Kruskal-Wallis test with Dunn’s multiple comparisons. c Representative images of HeLa cells on AS1411 MP surfaces showing brightfield, mechanosignal, mEGFP-paxillin, and phalloidin-stained actin. d Line profile from ( c ) shows intensity profiles of mechanosignals, mEGFP-paxillin, and actin. e Schematic showing the source of nucleolin-mediated forces. f Quantification of mean fluorescence intensity per cell following cytoskeletal inhibition. n = 72 cells from 3 replicates. Kruskal-Wallis test with Dunn’s multiple comparisons. g Representative brightfield and fluorescent images of HepG2 cells treated with internalization inhibitors on Sgc8 MP surfaces. h Corresponding quantification of mean fluorescence intensity per cell. n = 54 cells from 3 replicates. One-way ANOVA with Bonferroni post-hoc tests. i Representative images showing plasma membrane staining, mechanosignals, and brightfield of HepG2 cells incubated on Sgc8 MP surfaces. j Line profile from ( i ) reveals spatial colocalization of mechanosignals at the inner edge of membrane invaginations. k Schematic showing the source of PTK7-mediated forces. l Brightfield and fluorescence images of HepG2 cells treated with PI3K inhibitor LY294002 on Sgc8 MP surfaces. m Corresponding quantification of mean fluorescence intensity per cell for HepG2 cells. n = 54 cells from 3 replicates. Unpaired, two-tailed Student’s t-test. n Representative brightfield and fluorescence images of HeLa cells after N-WASP siRNA knockdown on AS1411 MP surfaces, and mean fluorescence intensity per cell. n = 85, 83 cells from 3 replicates for scrambled and siRNA groups. Unpaired two-tailed Student’s t-test. o Representative brightfield and fluorescence images of HepG2 cells after N-WASP siRNA knockdown on Sgc8 MP surfaces, and mean fluorescence intensity per cell. n = 45 cells from 3 replicates. Unpaired two-tailed Student’s t-test. All graphs, except ( d , j ), are presented as mean ± s.d. a.u., arbitrary units. Scale bar = 20 µm. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Synthetic aptamer mechanoreceptors enable cell-specific force sensing and temporal control via DNA circuits

    doi: 10.1038/s41467-026-70765-w

    Figure Lengend Snippet: a Representative brightfield and fluorescence images of HeLa cells treated with internalization inhibitors on AS1411 MP surfaces. b Corresponding quantification of mean fluorescence intensity per cell for HeLa cells. n = 70, 70, 54, 54 (left to right) cells from 3 replicates. Kruskal-Wallis test with Dunn’s multiple comparisons. c Representative images of HeLa cells on AS1411 MP surfaces showing brightfield, mechanosignal, mEGFP-paxillin, and phalloidin-stained actin. d Line profile from ( c ) shows intensity profiles of mechanosignals, mEGFP-paxillin, and actin. e Schematic showing the source of nucleolin-mediated forces. f Quantification of mean fluorescence intensity per cell following cytoskeletal inhibition. n = 72 cells from 3 replicates. Kruskal-Wallis test with Dunn’s multiple comparisons. g Representative brightfield and fluorescent images of HepG2 cells treated with internalization inhibitors on Sgc8 MP surfaces. h Corresponding quantification of mean fluorescence intensity per cell. n = 54 cells from 3 replicates. One-way ANOVA with Bonferroni post-hoc tests. i Representative images showing plasma membrane staining, mechanosignals, and brightfield of HepG2 cells incubated on Sgc8 MP surfaces. j Line profile from ( i ) reveals spatial colocalization of mechanosignals at the inner edge of membrane invaginations. k Schematic showing the source of PTK7-mediated forces. l Brightfield and fluorescence images of HepG2 cells treated with PI3K inhibitor LY294002 on Sgc8 MP surfaces. m Corresponding quantification of mean fluorescence intensity per cell for HepG2 cells. n = 54 cells from 3 replicates. Unpaired, two-tailed Student’s t-test. n Representative brightfield and fluorescence images of HeLa cells after N-WASP siRNA knockdown on AS1411 MP surfaces, and mean fluorescence intensity per cell. n = 85, 83 cells from 3 replicates for scrambled and siRNA groups. Unpaired two-tailed Student’s t-test. o Representative brightfield and fluorescence images of HepG2 cells after N-WASP siRNA knockdown on Sgc8 MP surfaces, and mean fluorescence intensity per cell. n = 45 cells from 3 replicates. Unpaired two-tailed Student’s t-test. All graphs, except ( d , j ), are presented as mean ± s.d. a.u., arbitrary units. Scale bar = 20 µm. Source data are provided as a Source Data file.

    Article Snippet: N-WASP antibody (C-1, sc-271484) was purchased from Santa Cruz.

    Techniques: Fluorescence, Staining, Inhibition, Clinical Proteomics, Membrane, Incubation, Two Tailed Test, Knockdown